▣ Title : Plasma Physics and Chemistry for

Biological Cell Interactions

               by Nonthermal Atmospheric Pressure Plasma

▣ Speaker

: Eun Ha Choi (Kwangwoon University)

▣ Date

& Time : Friday, June 13(2:00 ~ 3:30pm)

▣ Place

: LG Research Building, Room #101

▣ Host

: Prof. Jae Koo Lee (T.2083)

▣ Abstract :

Current

research activities of plasma bioscience and medicines in Plasma Bioscience

Research Center (PBRC), Korea, will be introduced along with the plasma physics

and chemistry for biological cell interactions. Basic diagnostics for electron

temperature and ion density have been introduced and measured to be ~ 1 eV and

~ 1×1013 cm-3, respectively, for the nonthermal atmospheric pressure dielectric barrier

discharge (DBD) plasma [1] and soft plasma jet [2] by atmospheric collisional

radiative model and wave-packet model[3]. Herein, we have also investigated the

basic generation mechanism of reactive oxygen species (ROS), especially for

hydroxyl radical OH species by ultraviolet absorption spectroscopy [4], and

their interactions with microbial [5] and mammalian cells resulting in

apoptotic cell death in accordance with the absolute densities of OH radical

species in biological solutions. Especially, differential selectivity of cell

death for epithelial human lung cancer cell H460 has been observed to be higher

than normal lung cell L132 in higher density of radical species, which is

caused by mitochondrial membrane potential decrease and enzymatic dysfunction

[1]. Similar effects of selective apoptosis were also observed with oral

squamous cell carcinoma, which the mechanism is suspected to be linked with

epidermal growth factor receptor (EGFR). Additionally, applications of

non-thermal plasma treatment on hard tissue regeneration have been considered

with regard to osteoblasts and mesenchymal stem cells. Recently, we also apply

DBD plasma and nitric oxide (NO) producing microwave plasma to enhance

differentiation and activation of microbial and mammalian cells. Furthermore,

we provide the possibility that nonthermal plasma jet could be developed as a

potential anti-diabetic therapy via ROS and NO dependent signaling pathway for

glucose uptake and insulin secretion [6].

References

[1]K. Pangomm, K. Y. Baik, M.K. Nam, J. H. Han, H. Rhim,

and E. H. Choi, Cell Death & Desease 4, e642 (2013).

[2]

P. Attri,

B. Arora, and E. H. Choi, RSC Advances 3, 12540 (2013).  

[3]

G. Cho, E. H. Choi, and H. S. Uhm, Phys. Plasmas 18,

034504 (2011).

[4]

Y.J. Hong, C.J. Nam, K.B. Song, G.S. Cho, H.S. Uhm, D.I. Choi and E.H. Choi, JINST 7,C03046 (2012).

[5]

Y.H. Ryu,

Y.H. Kim, J.Y. Lee, G.B. Shim, H.S. Uhm, G. Park, and E.H. Choi, PLOS One 8,

e66231 (2013).

[6]

N. Kumar, N. K. Kaushik, G. Park, E. H. Choi, and H. S. Uhm,

Appl. Phys. Lett.

103, 203701 (2013).